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KMID : 1234420080360040360
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 4 p.360 ~ p.365
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Evaluation and Improvement of Bioassay for Residual Antibiotics in Foods
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Park Min-Hee
Kim Tae-Woon
Jo Nam-Uk
Jeong Ji-Yoon
Lee Soon-Ho
Lee Jong-Ok
Kim Hae-Yeong
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Abstract
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For the screening of residual antibiotics in foods, bioassays and microbiological inhibitor tests are commonly applied. These methods are tested by the various susceptibility of bacteria against different kinds of antibiotics. However, the sensitivity of bioassay is generally insufficient to detect some residual antibiotics at level of interest. This study was performed to investigate the detection limit of variable antibiotics of the bioassay and to improve the sensitivity to some antibiotics. The sensitivity of bioassay using Bacillus megaterium ATCC 9885, B. subtilis ATCC 6633, B. cereus ATCC 11778 and Geobacillus stearothermophilus ATCC 10149 was low in the detection of macrolides, quinolones, chloramphenicol, and monensin. On the contrary, Micrococcus luteus ATCC 9341 showed high sensitivity to macrolides and Escherichia coli ATCC 11303 was highly sensitive to quinolones and aminoglycosides. Consequently, both strains would be useful to improve sensitivity of bioassay with a wide detection range.
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KEYWORD
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Bioassay, antibiotics, screening
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